Micro-immunotherapy (low dose immunotherapy) is an immunotherapy aimed at regulating the immune system by using immunomodulatory substances, mainly cytokines, in low doses. Its good safety profile and efficacy have been validated in multiple studies, as shown in the following table.

Year Authors Journal Title
2021 Jacques, C., Chatelais, M., Fekir, K., Fauconnier, L., Mellier, M., Togbe, D., Floris, I. International Journal of Molecular Sciences The Micro-Immunotherapy Medicine 2LEID Exhibits an Immunostimulant Effect by Boosting Both Innate and Adaptive Immune Responses
2021 Jacques, C., Lejeune, B., Floris, I. International Journal of Molecular Sciences Ultra-Low Dose Cytokines in Rheumatoid Arthritis, Three Birds with One Stone as the Rationale of the 2LARTH® Micro-Immunotherapy Treatment
2020 Ferrà, M., Munar, M. García, L. Lejeune, B. Ramis, J.M., Monjo, M. Journal of Periodontology BMP4 micro-immunotherapy increases collagen deposition and reduces PGE2 release in human gingival fibroblasts and increases tissue viability of engineered 3D gingiva under inflammatory conditions
2020 Floris, I., García-González, V., Palomares, B., Appel, K., Lejeune, B. International Journal of Rheumatology The Micro-Immunotherapy Medicine 2LARTH® Reduces Inflammation and Symptoms of Rheumatoid Arthritis In Vivo
2020 Floris, I., Chenuet P., Togbe D., Volteau C., Lejeune B. Dose Response Journal Potential Role of the Micro-Immunotherapy Medicine 2LALERG® in the Treatment of Pollen-Induced Allergic Inflammation
2020 Floris, I., Rose, T., Collado, J.A., Appel, K, Roesch, C., Lejeune, B. Dose Response Journal Pro-Inflamatory Cytokines at Ultra-Low Dose Exert Anti-Inflammatory Effect In Vitro: A Possible Mode of Action Involving Sub-Micron Particles?
2019 Lilli, N., Révy, D., Robelet, S., Lejeune, B. Degenerative Neurological and Neuromuscular Disease – Dove Press journal Effect of the micro-immunotherapy medicine 2LPARK® on rat primary dopaminergic neurons after 6-OHDA injury: oxidative stress and survival evaluation in an in vitro model of Parkinson’s disease
2019 Lilli, N., Révy, D., Volteau, C., Robelet, S., Lejeune, B. Biomedical & Life Sciences Journal Effect of 2LMISEN® on Long-Term Hippocampal Neurons Culture as a Screening Senescent Cells Model: p16INK4A and Caspase 3 Quantification
2018 Floris, I., Lechner, J., Lejeune, B. Journal of Biological Regulators & Homeostatic Agents Follow-up of patients with systemic immunological diseases undergoing fatty-degenerative osteolysis of the jawbone surgery and treated with RANTES 27CH
2018 Floris, I., Appel, K., Thorsten, R., Lejeune, B. Journal of Inflammation Research 2LARTH®, a micro-immunotherapy medicine exerts anti-inflammatory effects in vitro and reduces TNF-alpha and IL-1beta secretion
2018 Mancini, F., Milardi, D., Carfagna, P., Grande, G., Miranda, V., De Cicco, A., Ricciardi, D., Pontecorvi, A., Marana, R., De Cicco, F. International Immunopharmacology Low-dose SKA Progesterone and Interleukin-10 modulate the inflammatory pathway in endometriotic cell lines
2017 Marin-Martin, L.S., Giovannangeli, F., Bizzi, E., Massafra, U., Ballanti, E., Cassol, M., Migliore, A. GDrug Des Devel Ther An open randomized active-controlled clinical trial with low dose SKA cytokines versus DMARDs evaluating low disease activity maintenance in patients with rheumatoid arthritis
2017 Tessaro, I., Modina, S., Lodde, V., Sivelli., Franciosi, F., Terzachi, L., Luchini, P., Rumio, C., Luciano, A. Journal of Reproductive Infertility Ultra-Low Dose of Follicle Stimulating Hormone and Progesterone Attenuate the Severity of Polycystic Ovary Syndrome Features in a Hyperandrogenized Mouse Model
2017 Uberti, F., Bardelli, C., Morsanuto, V., Ghirlanda, S., Cochis A., Molinari, C. Cells, Tissues, Organs Stimulation of the Nonneuronal Cholinergic System by Highly Diluted Acetylcholine in Keratinocytes
2016 Camps, A., Almolda, B., Gonzalez, B., Castellano, B. Homeopathy Microimmunotherapeutic administration of cytokines improve the clinical symptoms in EAE an animal model of multiple sclerosis
2016 Barygina, V., Becatti, M., Lotti, T., Moretti, S., Taddei N., Fiorillo, C. Journal of Dermatological Science Low dose cytokines reduce oxidative stress in primary lesional fibroblasts obtained from psoriatic patients
2016 Fiorito, F., Cantiello, A., Granato, G.E., Navas, L., Diffidenti, C., De Martino, L., Maharajan, V., Olivieri, F., Pagnini, U., Lovane, G. Comp Immunol microbiol Infect Dis. Clinical improvement in feline herpesvirus 1 infected cats by oral low dose of interleukin-12 plus interferon-gamma
2016 Thomas, G., Cluzel, H., Lafon, J., Bruhwyl, J., Lejeune, B. Advances in Infectious Diseases Efficacy of 2LPAPI®, a Micro-Immunotherapy Drug, in Patients with High-Risk Papillomavirus Genital Infection
2015 Barygina, V., Becatti, M., Lotti, T., Moretti, S., Taddei N., Fiorillo, C. Journal of Dermatological Science Treatment with low-dese cytokines reduces oxidative-mediated injury in perilesional keratinocytes from vitiligo skin
2015 Lotti, T., Hercogovo, J., Wollina, U., Chokoeva, A.A., Zarrab, Z., Gianfaldoni, S., Roccia, M.G., Fioranelli, M., Tchernev, G. J Biol Regul Homeost Agents Vitiligo: successful combination treatment based on oral low dose cytokes and different topical treatments
2015 Radice, E., Bellone, G., Miranda, V. Translational Oncology Enhancement of the Immunostimulatory Functions of Ex Vivo–Generated Dendritic Cells from Early-Stage Colon Cancer Patients by Consecutive Exposure to Low Doses of Sequential-Kinetic-Activated IL-4 and IL-12. A Preliminary Study
2013 Cardani, D., Dusio, G., Luchini , P., Sciarabba, M., Solimene, U., Rumio, C. Gastroenterology Research Oral Administration of Interleukin-10 and Anti-IL-1 Antibody Ameliorates Experimental Intestinal Inflammation
2012 D’Amico, L., Ruffini, E., Ferracini, R., Roato, I. Journal of Cancer Therapy Low Dose of IL-12 Stimulates T Cell Response in Culters of PBMCs Derived from Non Small Cell Lung Cancer Patients
2012 Mazzoli, S., Cai, T., Meacci, F., Addonisio, P., Dorfman, P. International Journal of High Dilution Research High Risk Human Papillomavirus genital infections in asymptomatic population: effectiveness of Micro-Immunotherapy
2011 Van der Brempt, X., Cumps, J., Capieaux, E. Revue Française d’Allergologie Clinical efficacy of 2LALERG®, a new sublingual immunomodulatory treatment, in seasonal allergic rhinitis: A double-blind placebo-controlled study
2009 Gariboldi, S., Palazzo, M., Zanobbio, L., Dusio, G., Mauro, V., Solimene, U., Cardani, D., Mantovani, M., Rumio, C. Pulmonary Pharmacology & Therapeutics Low dose oral administration of cytokines for treatment of allergic asthma
2000 Jenaer, M., Henry, MF., García, A., Marichal, B. British Homeopathic Journal Evaluation of 2LHERP® in preventing recurrences of genital herpes